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Theworldwideintroductionofbio-dieselinrecentyearsinpursuitofconservingfossilfuelcarbonneutraltargetsandenergysecurityhasbroughtamarinerx19sfuelproblemonlandbio-dieselisaperfecthabitatforvigorousmicrobialgrowth.TheLloydsRegisterpublishedatechnicalpaperPaperN4Session1994-95highlightingcriticallevelsofbacteriainfuel.Lessthan500cfucolony-formingunitsperlitreisacceptable.Atthe500-1000cfulevelLloydswarnsx1cmicrobialproliferationoccurringx1dandatover1000cfux1cmicrobialproliferationoperationalproblemsinvestigatethoroughlyuseanti-microbialtreatmentx1d.Inpetroleumdieselthecriticallevelsbeyond500cfuwouldrarelybeseenoutsideofmarineorlongtermstoragesituations.Bio-dieselisadifferentmatterx13thehigherwatercontentprovidesthemicrobeswithaveryattractiveenvironment.Consequentlytheamountofexposuretocondensationleaksortimeinundisturbedstoragebeforeproliferationbeginsisdramaticallyreduced.Todayx19sbiofuelsdeterioratemuchfasterthantheirpetroleumforebears.ColonyFormingUnitsofbacteriagrowonthewater-fuelinterfacebecomingheavieruntiltheydropoutofthefueltothebottomofthetankwherebio-lmsandsludgequicklyform.Thebacteriafeedsonthefuelx13butthemicrobesdonoteatx18allx19ofthefueltheybreakdownthecarbonchainswhichreducesthecombustibleproperties.ThisleadstoPoorstartingorfailuretostartatall.Excessivesmoke.Reducedpoweroutput.x1cMICroBIALContAMInAtIonISnotLIMIteDtoDIeSeLx1dAviationjetfuelsaresubjecttothesameproblemswhicharethereasonfortheaviationindustryx19srigorousfuelmanagementregimes.Gasoline-petrol-hasuntiltheintroductionofbio-contentbeentheonlyfuelunaffectedbymicrobialcontaminationbutpetrolcontainingbio-ethanolsufferstoo.Universaluseofbiofuelsisatleastasprofoundachangetofuelsastheswitchtounleadedpetrolinthe1990x19s.Fuelqualitymanagementisnolongersomethingthatrestsonlywithreneriesshipownersandairlines.6